Describe the approach you would take and why, as well as the controls that you would use and what the expected outcome might be.

A new disease (make up a name for it if you like!) is thought to be associated with a mutation in a peptide targeting sequence, causing mis-localisation of a receptor protein to the nucleus instead of the plasma membrane. The gene encoding this receptor is known. You have cloned both the normal receptor gene from a control source and the mutated version of the gene from a patient with the disease. Now, how could you confirm whether the mutation causes mis-localisation of the receptor protein?
Describe the approach you would take and why, as well as the controls that you would use and what the expected outcome might be.

**Localization can be effectively demonstrated with fluorescence microscopy based technique

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