Instructions (read and follow all of the following instructions):
1. Read and complete all of Lab 12 Evolution and Population Genetics in the Lab Manual (pages 89 – 97), which you should have already done and completed both Sessions 1 and 2. Also, be sure to watch the recorded online lab lecture, required viewing for this lab as well as have access to the necessary freely available website for Lab 12 Population and Evolutionary Genetics: This Lab 12 Report will ONLY cover Evolution of the Bitter Taste Receptor, Session 1
(pages 89-92).
Websites you will need to use as you work through Lab 12 in Lab Manual Session 1:
National Center for Biotechnology Information (NCBI) www.ncbi.nlm.nih.gov Cold Spring Harbors Bioserver/Allele Server: www.bioservers.org
2. This is an individual student assignment. Your Lab Report and writing must be in your own words using proper, formal English in full paragraph, narrative format. Although key words and concepts will be similar between your Lab Report and the Lab Manual, do NOT copy/paste whole sentences/sections from the Lab Manual; that is plagiarism. Any evidence of cheating through group work, shared answers, plagiarism, etc, as determined by instructor, will result in points taken off, zero points for the Lab Report, or worse.
3. You will use Lab 12, Session 1 (Evolution of the Bitter Taste Receptor, Session 1, pages 89-92) to complete the lab activity and obtain data for this lab report. Follow the Lab Manual in Lab 12, Session 1 step-by-step. Also, be sure to review the Lab 12 Lab Lecture with background information, available in the Canvas Genetics course site.
4. Write Lab Report using the required format , which should be between 3 5 pages (max of 6), single-spaced, 12-point font. Submit your completed Lab Report online through the Canvas Assignment feature. It must be in one of the following computer file formats: MS Word .doc, .docx, or Acrobat .pdf file format. No other file format will be accepted.
5. Upload your Lab 12 Report file on or before Wednesday May 11, 2022, by 11:59 pm (or earlier if desired) on the Genetics course Canvas site. Be aware, I and Canvas will NOT accept assignments after due date and time. You will also have to agree to the tools End-User License Agreement and that this assignment is my own, original work.
Lab Report Format, Required Sections, and Anticipated Lengths:
1. Introduction (2 3 paragraphs, about 1 page): Describe the overall purpose of this lab for the Lab 12, Session 1 ONLY on the TAS2R38 gene and its evolution. Be sure to explain the different alleles and nature of the polymorphisms for TAS2R38 in humans and overview the general molecular genetic methods for TAS2R38 genotyping. Provide needed background for your report. Clearly write out experimental goals and purpose of Lab 12, Session 1 (TAS2R38 portion) and your hypothesis.
2. Methods (~2 long paragraphs, about half a page): Write a summary of the overall methods used. Note, do not just copy/paste the step-by-step details from the Lab Manual. Also, do NOT describe each individual step for each procedure, instead cite the Genetics Lab Manual and provide a general overview of the methods in your own words. Emphasize key aspects for the methods for this experiment. Keep in mind, bioinformatic software and online applications are also part of the methods that need to be included in your Methods section. This includes citing the exact websites used in your analysis. Use past-tense verbs.
3. Results (2 3 pages): Use your Lab 12, Session 1 processes and data obtained from it regarding evolution of the TAS2R38 gene among primates. The data will come from your bioinformatics analysis that you obtain through NCBI website (www.ncbi.nlm.nih.gov) and the Bioserver website (www.bioservers.org). The Results must also include a formally written text in narrative format that describes your results and that cites and describe the primary data you provide in your figure(s) and table(s). For any Figures, be sure to include a formal figure legend, which must be placed below the figure. For Tables, if any, the Legend is always shown at the top (above the table). Where appropriate, your results should include answers to the questions from the Lab Manual, pages 89-92, that are written into your Results section. As always, use proper units for all data. Pictures of hand-written answers or data from Lab Manual are NOT acceptable in a formal report. Instead, reproduce data and figures within MS Word, Powerpoint, or Excel documents. Keep in mind, you should NOT include every factoid or answer for every question in Lab 12 from the Lab Manual in your Lab Report. Use pasttense verbs.
4. Conclusion (~ half page to a page): Summarize your conclusions from these data, relative to the key biological questions and your hypothesis, from Introduction. This is a very important section and one you should be sure to refer back to your general goal and hypothesis from the Introduction. Do not just repeat and re-show the data already presented in the Results section. Instead use the key data and general conclusions about these data and results. Make datasupported summary statements about your findings that are relevant to the Introduction and your original hypothesis. Use present-tense verbs.
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