Would anyone like to examine the mechanisms for the necroptotic or pyroptotic pathways?

Would anyone like to examine the mechanisms for the necroptotic or pyroptotic pathways?
The intrinsic pathway for apoptosis starts with a internal signal, such as DNA damage. P53 then upregulates the expression of pro-apoptotic proteins. One group of pro-apoptotic proteins (the sensitizers BAD/NOXA/BIK) bind to and directly inhibit antiapoptotic proteins (BCL-2/BCL-XL). The antiapoptotic proteins are now inhibited and cannot bind to BAK/BAX. Activator proteins (BIM/BID/PUMA) are now able to bind to BAK/BAX and activate them. Once activated, BAK/BAX oligomerize and create a pore in the outer mitochondrial membrane. This pore allows cytochrome c to escape into the cytoplasm where it binds with Apaf-1. Apaf-1 binds to procaspase-9 and forms a complex called an apoptosome where procaspase-9 is cleaved into caspase-9.

When cytochrome c is released, two other proteins (Smac and HTRA2) are also released. Smac/HTRA2 binds to XIAP (which is an inhibitor of caspases 9 and 7). Active caspase 9 cleaves/activates caspases 3 and 7, which then activate other caspases along the signaling pathway (caspases 2, 6, 8, and 10) (refer to figure 3) [1].

In WT cells (with Apaf-1 present) and Apaf-1 deficient cells, apoptosis still occurred. The authors state these results show that apoptosis does not regulate the cell death pathway induced by TNF and INF [2]. Based on the mechanism I mentioned above, if there is no Apaf-1, then cytochrome c cannot bind to it and activate it, and Apaf-1 cannot bind and activate procaspase-9. In which case, one would think cell death would not occur through the apoptotic pathway. My thoughts are that cell death must have occurred from the necroptotic or pyroptotic pathways.

Would anyone like to examine the mechanisms for the necroptotic or pyroptotic pathways?

Source:

[1] Bertheloot, D., Latz, E., & Franklin, B. S. (2021). Necroptosis, pyroptosis and apoptosis: an intricate game of cell death. Cellular & molecular immunology, 18(5), 11061121. https://doi.org/10.1038/s41423-020-00630-3

[2] Karki, R., Sharma, B. R., Tuladhar, S., Williams, E. P., Zalduondo, L., Samir, P., Zheng, M., Sundaram, B., Banoth, B., Malireddi, R., Schreiner, P., Neale, G., Vogel, P., Webby, R., Jonsson, C. B., & Kanneganti, T. D. (2021). Synergism of TNF- and IFN- Triggers Inflammatory Cell Death, Tissue Damage, and Mortality in SARS-CoV-2 Infection and Cytokine Shock Syndromes. Cell, 184(1), 149168.e17. https://doi.org/10.1016/j.cell.2020.11.025

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