ONLY COMPLETE THE CHART IN THE ATTACHED DOCUMENT!!
Directions:
Login to
https://courses.hayden-mcneil.com/local/ecologin/
username: jnarcisse
password: Jnarcisse2022
1. Click on my courses
2. Click on school name and course name under course overview
3. On the right, click on cellular respiration, then green book.
4. Click on procedure beneath background
5. Click om blue box to start lab
6. Please use directions below to complete lab and the only the chart in the attached document.
Lab Directions
Fermentation of Different Sugars by Yeast Cells
1. Take a 150 mL Erlenmeyer flask from the Containers shelf and place it onto the workbench.
2. Take a thermometer from the Instruments shelf and add it to the Erlenmeyer flask. Switch the mode by pressing the blue button on the display until it shows a K for the Kelvin scale. Record the temperature in Kelvin to reference later.
3. Take a gas syringe from the Instruments shelf and place it onto the workbench, right next to the flask. Open the menu for the syringe by double-clicking on the syringe to read the initial gas volume. Record the volume in mL.
4. Take the 5% glucose solution from the Materials shelf and add 50 mL to the Erlenmeyer flask. Double-click on the Erlenmeyer flask and rename it 5% glucose.
5. Review the process in this step carefully before performing it. Several things need to be done very quickly in sequence to get accurate results.
o Take the 2% yeast solution from the Materials shelf and add 50 mL to the Erlenmeyer flask.
o Immediately after adding the 2% yeast solution, put the gas syringe onto the Erlenmeyer flask. You should see an black rubber stopper automatically appear to seal the flask.
o Immediately start a timer or note the time on the lab clock.
6. After 1 minute, press the lab pause button.
7. at the final volume of gas in the syringe and the temperature of the flask and record these values.
8. Press the lab play button to continue with the experiment.
9. Drag the syringe and thermometer back to the Instruments shelf. Empty the flask into the waste container, and then place the empty flask in the sink.
10. Repeat steps 1 9 with the 5% fructose, 5% maltose, and 5% maltotriose solutions replacing the 5% glucose solution.
11. Clear the bench of all materials, containers, and instruments, then return to your course page to complete any assignment for this lab.
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